The styrofoam CryoCaddy houses and transports the super cooled cryoblock. The caddy is coated with a urethane shell for durability, and the shell is impregnated with titanium oxide which kills surface bacteria when exposed to light.
Retrieved from an ultra low temperature freezer or maintained at -78C (-104F) with dry ice the cryoblock provides the thermal inertia to cool the chuck and freeze the embedding gel.
The cryoblock is machined from 6061 aluminium. The black anodized finish provides durability and an ideal background to visualizing freezing as the gel transitions from a colourless, transparent fluid to an opaque white solid.
The corner chuck post holes have the most thermal inertia when the cryoblock is cooled and surrounded with dry ice while the two central holes have the highest freezing capacity when the cryoblock is used straight out of an ultra low freezer.
The customized chucks have a surface for specimen labeling so the tissue and tracking information are never separated.
The oversized chuck face provides increased surface area for heat transfer, reducing freezing time to a minimum. The “target” etched into the chuck face is the size of a standard tissue processing cassette.
The disposable molds are used to embed frozen section specimens, which is similar to paraffin embedding. Like the chucks the embedding molds have a tab for specimen labelling.
The molds are designed to be sufficiently rigid at room temperature so they hold their shape, while being flexible enough at – 78C (-104F) so they don’t buckle or split. The bevelled sides allow for easy release of the block after freezing is complete.
The Heat Sinks are used on the inverted block face to prevent tissue thawing and the formation of ice crystal artefact. They also accelerate the freezing process. The blue fluorosilicon O ring and hole in the stem of the heat sink are there to facilitate safe handling.